Tree borne oil seeds (TBO’s) is one of the important group of Non Timber Forest Products (NTFP’s) having multifarious usage and industrial applications. Maharashtra state is a treasury for genetic and species diversity of TBO’s. In the present investigation morphophysiological studies of 9 TBO’s viz. Aphanamixis polystachya (Wall.) R. Parker, Holoptelea integrifolia Planch, Madhuca longifolia var. latifolia (Roxb.) A. Chev, Mesua ferrea L., Mimusops elengi L., Moringa oleifera Lam., Pongamia pinnata (L.) Pierre, Putranjiva roxburghii Wall, Ricinus communis L. collected from Western Ghats of Maharashtra were studied. Out of the studied TBO’s maximum oil content was recorded in M. ferrea (78.78%) followed by H. integrifolia (54.77%), and lowest in M. elengi (19.84%). Maximum 100 seed weight was observed in M. ferrea (336.8 gm) followed by M. longifolia (332.67 gm) and lowest in H. integrifolia (1.98gm). High fatty acids variation found in P. pinnata and M. oleifera. Oleic acid is found to be a dominant fatty acid in higher concentration in the seeds of studied TBO’s. This investigation also provides information about the oil quality and fatty acid composition. Its industrial applications and bioprospecting is to accessed very much for future use.