Seema Pal, Srishti Sharma, Surbhi, Deepshikha Kataria; IJGHC, December 2017 – February 2018; Sec. B; Vol.7, No.1, 067-074.;DOI: 10.24214/IJGHC/HC/7/1/06774
Background: The present study was done to investigate antioxidant properties and antimicrobial activity of leaves of Ficus religiosa. The antimicrobial activity of ethanolic extract of leaves of Ficus religiosa was analyzed against E.coli using well diffusion method. Total phenols, condensed tannins and total flavonoid content of ethanolic extract of leaves of Ficus religiosa were analyzed. The total polyphenols in ethanolic extract of leaves of Ficus religiosa were 21.55 ± 0.912 mg GAE g-1, condensed tannin content was 166.66 mg CE g-1 and total flavonoid content was found to be 704.66 mg CE g-1. Antioxidant activity of the extract was estimated using DPPH radical scavenging assay and was found to be 46.21%. Well diffusion method had shown zone of inhibition against E.coli. The study concluded that Ficus religiosa is a potent antioxidant and antimicrobial agent. It can be incorporated into various products and can be used as an antioxidant and antimicrobial agent in foods and pharmaceuticals.
K.N.Vinay, V. Venkata Lakshmi, N. D. Satyanarayan, Sunil More, Vineetha; IJGHC, December 2017 – February 2018; Sec. B; Vol.7, No.1, 057-066.;DOI: 10.24214/IJGHC/HC/7/1/05766
The objective of the study was to evaluate acetylcholinesterase (AChE) inhibitory activity of different crude extracts from alpinia purpurata as a new source for management of Alzheimer’s disease by Ellman’s microplate colorimetric method and antioxidant activity of this plant was measured by spectrophotometric method tested with three different concentrations (5, 50 and 100 μg).According to the obtained results, the order of inhibitory activity (IC50 ; μg/ml) of different solvent extracts from highest tolowest was methanol extract (19.08±0.02),n-hexane extract (21.93 ± 0.02) and dichloromethane extract (22.02± 0.05). The results indicated that the methanol extracts exhibited significant antioxidant activity when compared with standard Butylated hydroxyl anisole (BHA)at 5 and 100 μg concentrations, the dichloromethane extract found be very high at 100 μg concentration. Inhibition of acetylcholinesterase (AChE) is still believed as the important therapeutic approach against Alzheimer’s disease; hence result indicated and confirmed that the traditional use of this plant for management of Alzheimer’s disease, this alpinia purpurata methanol extract will be a better candidate for further investigations and identification of active components.
Mrs. Margi H. Patel, Dr. (Mrs.) Farida P. Minocheherhomji; IJGHC, December 2017 – February 2018; Sec. B; Vol.7, No.1, 049-056.;DOI: 10.24214/IJGHC/HC/7/1/04956
Herbal medicine refers to the use of any of the plant material like seeds, berries, roots, leaves, bark, or flowers for medicinal purposes. In the present study, methanolic extract of the leaves of Mallotus phillipensis, Kalanchoe integra and Aegle marmelos were investigated for antimicrobial activity using agar well diffusion method. For antimicrobial activity, five bacterial and two fungal strains were selected. The phytochemical screening of the leaves extract of all three plants revealed the presence of alkaloids, terpenoids, tannis, flavonoids, phenolic flavonoids and proteins. All the selected bacterial and fungal strains were susceptible to methanolic leaves extracts of Mallotus phillipensis, Kalanchoe integra and Aegle marmelos at varying degree. It is clear from the results of the present studies that the plant extracts have potential as antimicrobial compounds against bacteria and fungi. However, there is a need of further research to isolate the active ingredients from plant part (leaves) for further pharmacological evaluation.">
Abdel Azim Alsanousi, Abdel Rouf A. Abbas, Fatima A. B. Abdalla, Abdelmonem M. Abdellah; IJGHC, December 2017 – February 2018; Sec. B; Vol.7, No.1, 039-0048.;DOI: 10.24214/IJGHC/HC/7/1/03948.
The wide use of a Sudanese snuff, which locally called toombak, is thought to be the main cause of oral cancer. Using spectrophotometry methods, the study was evaluated the levels of each of enzyme lactate dehydrogenase, albumin and uric acid in both saliva and serum of toombak dippers. A group of 150 toombak dippers were compared with a group of 50 healthy control subjects and 50 patients with oral squamous cell carcinoma (OSCC). Result indicated that there was no significant difference found between levels of lactate dehydrogenase and uric acid in saliva and serum of the three groups. There was a significant increase of albumin in saliva and a significant decrease of albumin in serum oftoombak dippersand OSCCpatients comparing to control. It may conclude that level of albumin in both saliva and serum of toombak dippers could be used as a real diagnostic marker for early changes caused by toombak dipping.
Baiju, E.C., Asha A., Sneha J. and Athira T.;IJGHC, December 2017 – February 2018; Sec. B; Vol.7, No.1, 033-038.;DOI: 10.24214/IJGHC/HC/7/1/03338.
Ophiorrhiza (Rubiaceae) is a critically endangered genus of Western Ghats. An investigation was carried out to explore the in vitro antioxidant and antibacterial activities of Ophiorrhiza pectinata Arn. andOphiorrhiza mungos L. To measure antioxidant activity, DPPH assay was performed and disc diffusion method was adopted for antibacterial activity. The IC50 values of DPPH assay recorded for O. pectinataand O. mungos were 78 μg ml-1 and 62 μg ml-1, respectively. The crude extract of O. mungos and O. pectinata showed significant antibacterial activity against gram-positive and gram-negative bacteria. Thus, the study revealed that aqueous extracts of both Ophiorrhiza species had the potential to scavenge free radicals and to inhibit growth of pathogenic bacteria.