Currently Published Papers in Herbal Chemistry

Antihyperglycemic and Antihyperlipidemic Moderating Effect of Catharanthus roseus Sadabahar) Leaves in Type II Diabetic Mellitus Subjects

K. Sharma, Akansha, S. Choudhary, E.S. Chauhan,IJGHC, March 2018 – May 2018;DOI: 10.24214/IJGHC/HC/7/2/23947. Sec. B; Vol.7, No.2, 239-247.

Diabetes mellitus is one of the well-known significant disorders of the endocrine system. It is characterized by an abnormal increase in the glucose load. Catharanthus roseus is an important Ayurvedic medication in traditional medicine. It is potentially used in countries like India, South Africa, China and Malaysia for the healing of diabetes mellitus. Due to the great antidiabetic and hyperlipidemic potential of Catharanthus roseus, we hypothesized that the effect of leaves powder shows a significant decrease in the blood glucose and lipid levels. The first group treated with leaves powder of Catharanthus roseus 75mg/100g incorporated in baked khakre. The biochemical evaluations of blood sample of subjects were done. The blood samples were taken using glucometer. The proposed study was framed to look at the antidiabetic and antihyperlipidemic efficacy of leaves powder of Catharanthus roseus. The doses were administered at a rate of 75mg/100g incorporated in baked khakre and detrained the glucose transport system in 8 weeks. We conclude that there is a significant decrease in blood glucose and lipid parameters. The findings are very encouraging and greatly advocate its candidature for the design of a novel herbal drug to cure deadly diabetes

Phytochemical Screening and Antimicrobial Activity of Methanol Extract of Microtrichia Perotitii DC (Asteraceae) against Selected Micro-Organisms

Abdullahi, M. N .and Mustapha, A. O. ,IJGHC, March 2018 – May 2018; Sec. B; Vol.7, No.2, 230-.238,DOI: 10.24214/IJGHC/HC/7/2/23038

The resistance of micro-organisms against synthetic drugs has drawn the attention of the world to look for alternatives from plants (herbs). Microtrichia perotitii (Asteraceae) is an herb that can be exploited most especially since not much studies have been reported on it despites its uses for treating pain related diseases. In this study the phytochemical contents and anti-microbial properties of the herb were investigated. The herb was collected from Okene in Kogi State, Nigeria and was authenticated, prepared and extracted with methanol following standard methods. The preliminary phytochemical screening was carried out according standard protocols while antimicrobial screening was performed according to agar diffusion method as modified on hospital isolates of the species of streptococcus, staphylococcus, Escherichia coli, shigella and candida. The phytochemical screening of the methanol extract revealed the presence of alkaloids, flavonoids, tannins, saponins, terpenoids and cardiac glycosides while phlobatannins and anthraquinones were not detected. Tannins, flavonoids and alkaloids are compounds with anti-microbial activities. Hexane, ethylacetate and methanol soluble fractions showed highest susceptibility against the species of streptococcus, Escherichia coli and only ethylacetate and methanol suppressed the growth of candida species. The methanol extract of Microtrichia perotitii possesses antimicrobial activity which could be described as antibacterial and antifungal owing to the type of phytocompounds present. Thus, Microtrichia perotitii represents a promising source of antibiotics as well enormous therapeutic potentials. This research is the first time it would be reported for Microtrichia perotitii.

Antimicrobial potential of Taraxacum officinale F.H. Wigg clonally propagated via organogenesis

Burcu ÇETİN, Fatih KALYONCU, Tülin Ezgi ÖZEN,IJGHC, March 2018 – May 2018; Sec. B; Vol.7, No.2, 224-230.DOI: 10.24214/IJGHC/HC/7/2/22429.

In this study, the antimicrobial effects of plantlets micropropagated by organogenesis method from leaf explants of T. officinale were investigated. T. officinale seeds were germinated in 30 ml Murashige and Skoog (MS) medium. The leaves excised from four-weeks-old germinating seedling was used as explants. The leaf explants was cut into 2 cm2 segments and transferred on MS medium supplemented with 2 mg L-1 benzil amino purine (BAP) and 2 mg L-1 indole  utric acid (IBA) for organogenesis studies. Obtained shoots were subcultured twice every 4 weeks. The shoots were harvested and dried at 24±2°C room temperature. For  antimicrobial studies, T. officinale cultivated by tissue culture was tested for its antibacterial activities by using agar well diffusion method. Ethanol and chloroform extracts from this plant were assayed against nine bacteria species (Staphylococcus aureus ATCC 6538, Escherichia coli ATCC 25922, Bacillus cereus ATCC 7064, Bacillus subtilis ATCC 6633, Salmonella typhimurium CCM 5445, Proteus vulgaris ATCC 6896, Enterococcus faecalis ATCC 29212, Enterobacter cloacae ATCC 13047, and Kocuria rhizophila ATCC 9341). Seed germination of T. officinale was observed in 10 days. Adventitious shoot regeneration was obtained at the end of the third week on leafweeks the shoots were harvested and dried for antimicrobial studies. As a result, ethanol extracts of T. officinale showed 21 mm and 23 mm inhibition zones against Escherichia coli and Proteus vulgaris, respectively. T. officinale was found weakly effective on gram negative bacteria explants at MS medium supplemented with 2 mg L-1 BAP and 2 mg L-1 IBA. After 8.weeks the shoots were harvested and dried for antimicrobial studies. As a result, ethanol extracts of T. officinale showed 21 mm and 23 mm inhibition zones against Escherichia coli and Proteus vulgaris, respectively. T. officinale was found weakly effective on gram negative bacteria.

Effect of Grape Seeds Extract on Attenuation of Aflatoxins Toxicity in Rats

Gihan M. Hammoud, Yasser M. Abd El-Shafea, Asmaa A. Salem, and Nivin S. Nail,IJGHC, March 2018 – May 2018; Sec. B; Vol.7, No.2, 204-223.DOI: 10.24214/IJGHC/HC/7/2/20423.

Aflatoxins (AFs) are groups of structurally related potent mycotoxins fungal metabolites that contaminate food and feed. AFs cause economical losses and adversely affect animals and human health. Moreover AFs consider as a serious hepatotoxic, carcinogenic, mutagenic and teratogenic agents. Grape seeds extract (GSE) is nutritionally rich natural herbal substance have substantial content of polyphenols. The present study aimed to evaluate biological role of GSE against AFs toxicity in rats. Aflatoxicosis was induced by treatment of rats with 1 and 2mg AFs/ kg body weight (b.w)/once/week for 3 and 6 weeks and characterized by significant dose and time-dependant increase of serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), gamma glutamyl transpeptidase (GGT), alkaline phosphatase (ALP), creatinine, urea, total cholesterol, triglycerides, glucose and plasma lipid peroxidation product (malondialdehyde, MDA) and significant dose and time-dependant reduction of serum albumin, total protein (TP) and plasma reduced glutathione (GSH). Parallel to changes in biochemical parameters, AFs- intoxicated rats showed dose dependant pathological changes in liver, kidney, testes and brain. The oral administration of GSE (150 mg/Kg b.w/daily) along with AFs was significantly attenuate toxic effects of AFs and enhance of antioxidant and metabolic systems of rats. GSE restore most of liver functions and kidney functions parameters of low AFs treated rats to normal and succeed to preserve architecture structure of tested organs. Therefore GSE could be considering as effective natural agent for controlling AFs toxicity especially at low dose.

Nutritional Value of Ocimum Sanctum

Sudeshna Biswas, Sufia Zaman and Abhijit Mitra; IJGHC, March 2018 – May 2018; Sec. B; Vol.7, No.2, 200-203.; DOI: 10.24214/IJGHC/HC/7/2/20003.

The leaves of Ocimum sanctum were analysed for its proximate and mineral composition. Fresh leaves of tulsi were collected from Sonamukhi region (23°18'27.27"N and 87°24'56.86"E) of Bankura district in West Bengal from different patches. The proximate and mineral composition of the leaves of O. sanctum was analyzed using standard methods of food analysis. The results show high carbohydrate content in the leaves with considerable protein content and low percentage of fat. Mineral composition indicated that the leaves of the species are good sources of K, Ca and Mg. High K content in the leaves suggest the plant to be highly beneficial for patients having blood pressure and hypertension.