Research Paper in Herbal Chemistry

Physicochemical, Phytochemical Antioxidant activity and Toxicity study of cissus quadrangularis  

Parul Singh and Asheesh Singh,DOI: 10.24214/IJGHC/HC/8/2/05166

The present research work is aimed to investigate the Pharmacognostical, preliminary, phytochemical and Antioxidant activity of cissus quadrangularis stem. The stems of Cissus quadrangularis Linn (Vitaceae) are reported to have great medicinal value. The whole plant is used in India for the treatment of various diseases. Antioxidant potential of the methanol extract of the leaves of Cissus quadrangularis Linn was evaluated by using 1, 1-diphenyl-2-picrylhydrazyl (DPPH) scavenging assay, reducing power and total antioxidant capacity. The extract showed significant activities in all antioxidant assays compared to the reference antioxidant ascorbic acid in a dose dependent manner. In DPPH scavenging assay the IC50 (The half maximal inhibitory concentration) value of the extract was found to be 87.5μg/ml while the IC50 value of the reference standard ascorbic acid was 59.26μg/ml. Total antioxidant activity was also found to increase in a dose dependent manner. Moreover, Cissus quadrangularis extract showed strong reducing power.

Isolation of Octacosane-10-ol-21-ene from Thujaoccidentalis L Cone and its Antibacterial Activity

Amitava Mandal, Goutam Kumar Basak, Ankana Karmakar, Tanmay Chowdhury, Susama Chakraborty, Rajib Biswas, Pranab Ghosh;DOI: 10.24214/IJGHC/HC/8/2 /06772

A phytochemical investigation of the cones of Thuja occidentalis L was carried out in order to isolate the secondary metabolites present in it. The main objective of the present study is to find out the active constituents that are responsible for its traditional use against human ailments. Octacosane-10-ol-21-ene was isolated from the ethyl acetate fraction in pure form. Structure elucidation was carried out based on spectral data (UV, IR and NMR). Antibacterial activity of the isolated compound was determined and both the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were evaluated against some Gram positive and Gram negative bacterial strains.

Evaluation of Phytochemical analysis of Chloroxylon swietenia DC. Leaves with TLC & HPTLC profiling of its extracts

Rajesh E, Selvaraju A, Gopalakrishnan M, Priya G & Sekar T.;DOI: 10.24214/IJGHC/HC/8/2/07381.

In this present study we carried out investigation of phytochemical constituents, thin layer chromatographic (TLC) and High performance thin-layer chromatographic (HPTLC) studies of Chloroxylon swietenia leaf extract by following standard protocol. The qualitative phytochemical analysis of different solvent in leaf extracts showed the presence of number of constituents in hexane, chloroform, ethanol, ethyl acetate and aqueous extract i. e: carbohydrate, flavonoids, alkaloids, quinones, coumarin, cardiac glycosides, for the TLC, new solvent system developed for the best separation of the phytoconstituents present in the extract. The solvent system selected for the best results of TLC was the ratio of Toluene: Ethyl acetate (5:1.5) for ethanolic extract and it gives the Rf values of the same. For 10µl concentration, 13 spots were observed at 254 nm, 8 spots were observed at 366 nm and 7 spots were observed derivatization with vanillin- Sulphuric acid. HPTLC studies were carried out as per the methods of Harborne and Wagner et al (1998). The profile of various individual secondary metabolites were made and developed for authentication. This profile was performed at 254nm and 15 peaks were observed. Out of 15 peaks, two peaks showed maximum peak area i.e. 6th peak with Rf 0.24 value 12.30% of peak area and 10th peak with Rf 0.64 value of 14.01% of peak. HPTLC finger print analysis developed to help in proper identification and quantification of marker compounds.

Antidiabetic activity of leaves extract of Hippophae rhamnoides L. in Streptozo tocin-nicotinamide induced type-II diabetes in rats

Neha Viyas, Rinki Kumari, G.P.I. Singh, Rajesh Dubey, Mehul Bhandari, and, G.P. Dubey;DOI: 10.24214/IJGHC/HC/8/2/08290

To evaluate the anti-diabetic activity of alcoholic leaves extract of SBT in streptozotocin-nicotinamide induced type-II diabetes in rats. Streptozotocin-nicotinamide induced type-II diabetic rats (n = 6) were administered alcoholic leaves extract of SBT(250 and 500 mg/kg, p.o.) or vehicle (0.5%CMC) or standard drug glibenclamide (0.25 mg/kg) for 21 days. Blood samples were collected by retro-orbital puncture and were analyzed for serum glucose by using glucose oxidaseperoxidase reactive strips and a glucometer. For oral glucose tolerance test, glucose (2 g/kg, p.o.) was administered to nondiabetic control rats and the rats treated with glibenclamide (10 mg/kg, p.o.) and aqueous leaf extract of SBT. The serum glucose levels were analyzed at 0, 30, 60,90 and 120 min after drug administration. The effect of the extract on the body weight of the diabetic rats was also observed.The alcoholic leaves extract of SBT(250 and 500 mg/kg, p.o.) induced significant reduction (P < 0.05) of fasting blood glucose levels in streptozotocin-nicotinamide induced type-II diabetic rats. In the oral glucose tolerance test, the extract decreased the homocysteine and maintain the body weight of diabetic rats.It is concluded that aqueous leaf extract of SBT has significant antidiabetic activity as it lowers the fasting blood sugar level in diabetic rats and increases the glucose tolerance and also decreased plasma homocystein.