The total objective of our study must be to identify drugs to estimate human illness by a thorough analysis of plant Ayurveda and modern medicine techniques must be coupled in order to bring out high quality of herbal product with rapid onset of action and good bioavailability. Aim: The aim of the study is to investigate Phytochemical screening of ethanolic extract of Shorea rebusta (gaertn).: The qualitative phytochemical analysis of Ethanolic : Aqueous extract (50:50) of Shorea robusta. was done by preliminary identification of bioactive compounds such as alkaloids, glycosides, Cardiac Glycosides, flavonoids, carbohydrate,tannins, are present. The results of % of  yield were showed the Ethanolic extract in a 12.21%w/w and extract of  Aqueous extract has 8.75%w/w respectively. IC₅₀ for standard Ascorbic acid was found to be 20.56 and Shorea robusta for was found to be 63.33.Thus the anti-oxidant activity of sample was less than that of standard ascorbic acid. Ethanolic extract of leaf showed significant antimicrobial activity against the selected microbes . The methanol leaf extract has maximum activity against Staphylococcus aureus, moderate activity against Escherichia coli , at concentration 200 μg. The results depict the time taken for paralysis and death of earthworms after the treatment with the test extracts at the selected concentrations. The data revealed that the aqueous extract has a better wormicidal effect than ethanolic extract with compared with the standard drug as Albendazole.

The anti-microbial properties of Panchapallava Kashaya (PPK) and Panchapallava Arka (PPA), formulations derived from five medicinal plants, were assessed against a range of microorganisms including Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, Streptococcus pyogenes, Candida albicans, and Aspergillus nizer using the agar cup diffusion method. The zone of inhibition (ZOI) was measured at different concentrations of the test and standard samples. With rising anti-microbial resistance, there is a need for effective, natural alternatives. Medicinal plant-based formulations like PPK and PPA offer promising solutions against bacterial and fungal pathogens. Both formulations exhibited antimicrobial activity, with increased zone of inhibition at higher concentrations. At a concentration of 5 mg/ml, neither PPK nor PPA showed any significant anti-microbial effect. However, at higher concentrations both samples demonstrated notable anti-microbial activity, with the 250 mg/ml concentration comparable to that of the standard drug at 5 mg/ml. The antibacterial activity of both formulations was found to be superior to their antifungal activity. The results suggest that the bioactive compounds, including tannins, flavonoids, alkaloids, and essential oils present in the medicinal plants, contribute to the anti-microbial effects observed. These findings highlight the potential of Panchapallava Kashaya and Panchapallava Arka as effective anti-microbial agents, particularly against bacterial pathogens.