Research Paper in Herbal Chemistry

Cancer treatment through Ayurveda: what should be the research protocol and research model

Dr. Rajeev Kumar , Prof. (Dr) Sunil Kumar Joshi , Dr Udai Narain Pandey, Dr Prabhat Kumar Verma,DOI: 10.24214/IJGHC/HC/13/2/10107.

Tumour is abnormal mass of tissue having a pathological disorder of cell growth. Its Characteristics are disproportionate and atypical proliferation of cells.  There are two types of tumours, first benign and another malignant. In second type cells are anomalous and can grow without any control. This second type is known as cancerous cells or malignant tumour. Treating this cancer are still is not possible in modern era. Multimodule therapy has been used in cancer treatment. Ayurveda, the ancient medical system has described incurable form of arbuda, that is malignant lesion. In ancient texts multiple treatments, guidelines, and therapy has been described for this. But it is very difficult to categorise the cancer patients for ayurvedic protocol. It needs a research protocol as well as proper research proforma and newer guidelines.  Here an attempt has been done to establish research protocol for cancer treatment in ayurveda

A Critical Review of Jeevaniya Gana and its Therapeutic uses Mentioned in Bruhatrayee

Dr.Priya Chandrakumari and Dr.Amirthesh.K;DOI: 10.24214/IJGHC/HC/13/2/10830.

Jeevaniya Gana means medicines that are good for our health. Acharya Charaka said that Jeevaniya gana is the most important group of ten herbs because it helps to keep people healthy and treat different diseases. Acharya Charaka and Vagbhatta made a list of ten medicines that can be used to improve health and nourish the body. These herbs mostly grow in the Himalayas, so you can only find them in a few places. Jeevaniya Gana is very important for the body because it gives us energy, helps us stay alive, and helps our body heal. It's like nutrients and boosts our immunity. Some research has been done to find medicinal plants in the Jeevaniya Gana group, but more work is needed to find the exact plants in this group. This article is about the classification and health benefits of these Jeevaniya Gana plants. 

Reversion of bacterial resistance of Ocimum gratissimum (Lamiaceae) on bacterial strains isolated from chronic wounds diagnosed in the district of Ouinhi in Benin

Hervé Kouessivi Janvier BOKOSSA, Armelle Sabine Yélignan HOUNKPATIN, Parfait DJOSSOU, Rafiatou BA, Dagbédji Damien TOFFA, Carine ZINSOU, Wilfrid Hinnoutondji KPETEHOTO, Zinsou Franck Maurille MIGNANWANDE, Flora HOUNDJREBO, Roch Christian JOHNSON,DOI: 10.24214/IJGHC/HC/13/2/13146.

Ocimum gratissimum, a species with antibacterial properties, is widely used empirically to treat bacterial infections by local populations in Benin. In modern medicine, antibiotics inhibit or destroy bacteria. Their excessive use has led to the emergence of resistant strains. The aim of the present study is to assess the reversion of bacterial resistance of Ocimum gratissimum in combination with conventional antibiotics on strains isolated from chronic wounds diagnosed in the Ouinhi populations. The susceptibility of these bacterial strains to conventional antibiotics was tested by solid-state antibiotic susceptibility testing. The diameters of inhibition observed with the antibiotic discs were assessed according to the reading scale of the Antibiogram Committee of the French Society of Microbiology (CASFM). This made it possible to identify some ineffective antibiotics on these bacterial strains. The next step was to evaluate the combined effect of an aqueous mixture of Ocimum gratissimum and the usual antibiotics on multi-resistant bacterial strains at concentrations below MIC values, using the microdillution method. The test was carried out in two stages: determination of the MICs of four standard antibiotics, and evaluation of the synergistic action of antibiotics and the aqueous mixture of Ocimum gratissimum. The modulation factor is based on the Fractional Inhibitory Concentration (FIC) defined by the ratio MIC (extract + ATB)/MIC(ATB).At the end of this study, it was found that in contact with the Mixture-Antibiotic solution, the MIC values of this combination recorded ranged from 78.10-4 mg.ml-1  ≤ MIC(Mixture-ATB) ≤ 1 mg.ml-1 in comparison to the singular action of the aqueous mixture of Ocimum gratissimum whose observed values ranged from 78.10-2 mg.ml-1  ≤ MIC(Mixture) ≤ 25mg.ml-1 or those of the four conventional antibiotics (Amoxicillin / AMC; Ciprofloxacin / CIP; Cotrimoxazole SXT; Erythromycin / ERI) which oscillate around 78.10-4 mg.ml-1  ≤ MIC(Conventional ATB) ≤ 1mg.ml-1 . What's more, the combined Extract-ATB effect on all germs tested is synergistic (CFI ˂ 0.5). The possible production of Improved Traditional Medicines (ITM) from this combination of the aqueous mixture Ocimum gratissimum and conventional antibiotics can be recommended.

Study of the efficacy of oil extracted from the soursop "Annona muricata" on adults of the "Bactrocera dorsalis" fruit fly"

Ndeye Bineta CAMARA, El Hadji Gorgui DIOUF, Malick MBENGUE, Mamadou Kébé, Mamadou Latyr Ndour, Adama Faye, Codou Guèye MAR DIOP,DOI: 10.24214/IJGHC/HC/13/2/14761

The data were analyzed using the "General Linear Model" procedure using Minitab 19.1.1 software. The factors studied were: time, number of dead insects, sex, feeding and their interactions. The results of the statistical analysis show that the spray tests give a better mortality rate when the flies' diet is 10 times less sweet.

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Evidence of the effect of essential oils on oral squamous cell carcinoma tumor cell viability

Grace Celis, Daniela Tobar, Rodrigo Cabello, Alfredo Molina- Berríos, Mario Díaz-Dosque and José A. Jara,DOI: 10.24214/IJGHC/HC/13/2/16274

Cancer is one of the leading causes of death worldwide, characterized by ineffective treatments approaches and increasing resistance to conventional chemotherapeutics. Considering these challenges, this study aims to assess the potential of essential oils as a therapeutic alternative. The research specifically focuses on investigating the cytotoxic effect in tumor cells within in vitro and in vivo models when essential oils are employed as a therapy in comparison to traditional drugs for managing oral cancer. Comprehensive search was conducted across various scientific databases, resulting in the inclusion of 16 studies within this systematic review. The gathered data strongly suggest that essential oils exhibit significant efficacy in inhibiting cell viability, particularly when utilized as a substitute therapy for oral cancer. These essential oils display varying levels of effectiveness, with observed inhibition rates ranging from 48% to an impressive 99.3%. Notably, certain essential oils have shown remarkable potential, achieving an outstanding 99.3% cell viability inhibition. In summary, the findings of this study underscore the substantial impact of utilizing essential oils as either a monotherapy or combination therapy in the treatment of oral cancer. While they may not achieve 100% inhibition, essential oils hold great promise in advancing the fight against cancer.