Ayurveda has a unique concept known as polyherbalism, albeit it can be difficult to define in terms of contemporary standards. The Sarangdhar Samhita, a work of Ayurvedic literature, introduced the concept of polyherbalism to achieve better medicinal efficacy. The amount of active ingredients employed from each plant is insufficient to provide any pharmacological impact. There is evidence to suggest that crude plant extracts frequently have higher potencies than individual ingredients. Due to synergism, polyherbalism confers some benefits which are not accessible in single herbal formulations. This work is to report the method of preparation of Antidiabetic polyherbal formulation, a novel polyherbal formulation for diabetic mellitus, and to find out the active principles in the formulation responsible for the antidiabetic action. To validate the antioxidant activity of the formulation and to study the mechanism of action of the phytochemical constituents in the formulation. The polyherbal formulation is made from the ethanol extract of Gymnema sylvestre, Berberis aristate, Costus speciosa, Pterocarpus marsupium, Syzygium cumini, Picrorrhiza kurroa, Trigonella foenum, Cinnamomum verum, Alstonia scholaris and Asphaltum pure. Fingerprinting of phytochemical constituents of the antidiabetic polyherbal formulation was performed using spectroscopical (like IR and UV) and chromatographic techniques like LCMS. Biochemical assays like DPPH and SOD radical scavenging assays were done to validate its biological activity. The mode of action of phytochemical constituents against monoamine oxidase B that have a high impact on diabetes management and complications was also investigated using molecular docking. LC-MS/MS analysis of antidiabetic polyherbal formulation ethanolic extract identified 33 compounds, including the popular flavonoids and phenolic acids. Among them, 9 compounds show antidiabetic activity. Besides this, these compounds also show biological activities like anticancerous, anti-inflammatory, antiaging, antiproliferative properties, etc. The absorption bands in the UV spectra and harmonic vibrations in the IR region indicate the presence of phytochemicals like carbohydrates, terpenoids, flavonoids, saponins, and alkaloids. The dose-dependent response of the anti-diabetic polyherbal formulation demands 40 µg/mL concentration of the drug for 50% DPPH radical scavenging and 10 µg/mL for SOD radical scavenging. The docking result points out that many of the active principles in the polyherbal formulation show a significant binding affinity score than the inbuilt ligand, safinamide binding affinity value, the inbuilt ligand safinamide had exhibited a docking score of -9.014 with -74.785 kcal binding energy. While pulmatin showed a docking score of  -10.533 with a binding energy of-64.220 kcal, genistein 8-C-glucoside with a docking score of  -9.541 with a binding energy of 64.673 kcal and all others have a comparable docking score and binding affinity. These results point out that the polyherbal formulation is rich in antioxidant ingredients and will require less amount for high performance. The findings obtained in the present work indicate that the novel antidiabetic polyherbal formulation, may constitute a safe multi-target remedy to treat diabetes.

Piliostigma thonningii is a medicinal plant of the Fabaceae family. The young unopened leaves of Piliostigma thonningii are used for the treatment of various diseases. This study aims to evaluate the antioxidant activity of the extract and fractions of young unopened leaves of Piliostigma thonningii. Successive fractionation of the hydroalcoholic extract yielded the hexane, dichloromethane, ethyl acetate, ethanol and aqueous fraction. The antioxidant capacity of the hydroalcoholic extract and fractions was carried out using the DPPH and electrochemical (DPV) method.  For the DPPH method, the ethyl acetate fraction (IC₅₀ = 0.07 ± 0.02 mg/mL) has a better antioxidant activity with DPPH followed by the hydroalcoholic extract (IC₅₀ = 0.08 ± 0.02 mg/mL) and dichloromethane fraction (IC₅₀ = 0.50 ± 0.20 mg/mL). Concerning the electrochemical method, the ethyl acetate fraction has good antioxidant activity (IC₅₀ = 0.273 ± 0.002 mg/mL) followed by the hydroalcoholic extract (IC₅₀ = 0.275 ± 0.001 mg/mL) and the dichloromethane fraction (IC₅₀ = 0.314 ± 0.004 mg/mL).

An herb that is anti-oxidant, lowers cholesterol and blood pressure, and helps with diabetes all at once – Terminalia arjuna, it is. Since ancient times, medicinal plants have been a significant source of therapeutic compounds used to treat human ailments. Ayurveda, Siddha, and Unani are just a few of the indigenous medical systems in India that utilize the Terminalia arjuna medicinal plant. The Combretaceae family tree known as the Arjuna (Terminalia arjuna Roxb) is a common sight throughout India. The majority of experimental and clinical research have revealed that the bark of T. arjuna has anti-ischemic, antihyperlipidemic and antioxidant properties. Triterpenoids, flavonoids, glycosides, tannins, phenolics, and arjunolic acid are some of its beneficial phytoconstituents. Experimental research has shown that the bark of T. arjuna has strong cardioprotective and potent antioxidant effects. This in-depth analysis covers numerous facets of its phytochemical, pharmacognostical, pharmacological, and clinical value to various disorders, particularly cardiovascular problems.

Shalya Tantra is one of the most important branch of Ayurveda, which describes surgical and para-surgical methods for treating a variety of illnesses. The goal of a surgeon is to remove or repair damaged structures, reduce wound complications, and use the healing process to restore functions.  A controlled diet and integrated wound care can increase life expectancy and provide a better prognosis. Aahara-vihara that is nourishing and healthy for the body as well as bringing happiness to the mind is known as pathya, while the contrary is known as apathya. A healthy, balanced diet is crucial for wound healing. As a result, Acharya Sushruta discussed the unique Aahara and vihara for the vrana sufferer.

Cancer is the most widespread and deadly disease that is on the rise nowadays as a result of our way of life.  Cancer is curable if it is diagnosed at an early stage, which means cancer without metastasis. There are a number of treatments available now in allopathy, like chemotherapy, radiation therapy and immunotherapy. But these therapies like chemotherapy, target cells that are dividing rapidly. These cells have a high rate of growth and multiplication. They are cells of the bone marrow, hair, epithelial lining, reticuloendothelial system and gonads. So, we need an Ayurvedic approach to combat these complications. Here we critically analyze which therapy opted for malignancy.